TY  - JOUR
AU  - Li, Yan
AU  - Xing, Wen
AU  - He, Yong-Zheng
AU  - Chen, Shi
AU  - Rhodes, Steven D.
AU  - Yuan, Jin
AU  - Zhou, Yuan
AU  - Shi, Jun
AU  - Bai, Jie
AU  - Zhang, Feng-Kui
AU  - Yuan, Wei-Ping
AU  - Cheng, Tao
AU  - Xu, Ming-Jiang
AU  - Yang, Feng-Chun
PY  - 2014
TI  - Interleukin 8/KC enhances G-CSF induced hematopoietic stem/ progenitor cell mobilization in Fancg deficient mice
JF  - Stem Cell Investigation; Vol 1, No 10 (October 2014): Stem Cell Investigation
Y2  - 2014
KW  - 
N2  - Background: Fanconi anemia (FA) is a heterogeneous genetic disorder characterized by a progressive bone marrow aplasia, chromosomal instability, and acquisition of malignancies. Successful hematopoietic cell transplantation (HCT) for FA patients is challenging due to hypersensitivity to DNA alkylating agents and irradiation of FA patients. Early mobilization of autologous stem cells from the bone marrow has been thought to be ideal prior to the onset of bone marrow failure, which often occurs during childhood. However, the markedly decreased response of FA hematopoietic stem cells to granulocyte colony-stimulating factor (G-CSF) is circumventive of this autologous HCT approach. To-date, the mechanism for defective stem cell mobilization in G-CSF treated FA patients remains unclear.  Methods: Fancg heterozygous (Fancg+/–) mice utilized in these studies. Student’s t-test and one-way ANOVA were used to evaluate statistical differences between WT and Fancg–/– cells. Statistical significance was defined as P values less than 0.05.  Results: Fancg deficient (Fancg–/–) mesenchymal stem/progenitor cells (MSPCs) produce significant lower levels of KC, an interleukin-8 (IL-8) related chemoattractant protein in rodents, as compared to wild type cells. Combinatorial administration of KC and G-CSF significantly increased the mobilization of hematopoietic stem/progenitor cells (HSPCs) in Fancg–/– mice.  Conclusions: In summary, our results suggest that KC/IL-8 could be proved useful in the synergistic mobilization of FA HSPCs in combination with G-CSF.
UR  - https://sci.amegroups.org/article/view/4965